Simultaneous Estimation of Zolmitriptan and Sumatriptan Succinate in Pure and Synthetic Mixture Using UV Spectrophotometer

Download PDF

A novel analytical method was developed and validated for the simultaneous estimation of Zolmitriptan and Sumatriptan succinate in bulk and synthetic mixture using a UV-spectrophotometer. For the wavelength selection of drugs, 0.1 N HCl was optimized and spectra were monitored at 226 nm and 282 nm respectively using Shimadzu UV-1800. The linearity was found in the concentration range of 2 -14 μg/mL for both Zolmitriptan (R² = 0.999) and Sumatriptan succinate (R² = 0.998) with an accepted regression value. The percentage recovery (80%, 100%, and 120% levels) of reference standards of Zolmitriptan and Sumatriptan succinate using the proposed method was found to be 99.1% - 99.8% w/w and for the synthetic mixture was found to be in the range of 99% - 99.8% w/w. Percentage RSD in intermediate precession of Zolmitriptan and Sumatriptan succinate was found to be 0.07 and 0.47 (intra-day), 0.05 and 0.39 (inter-day). The percentage drug content of Zolmitriptan and Sumatriptan succinate in a synthetic mixture was found to be 100.42 ± 0.02% and 103.90 ± 1.02%. Furthermore, the degradation study for the mixture of Zolmitriptan and Sumatriptan succinate and synthetic mixture under acidic, basic, thermal, and photolytic conditions except peroxide degradation were within the acceptance limits. The proposed method was successfully validated by the ICH Q2 R1 guideline and was found to be specific, accurate, precise, robust, and rugged for the simultaneous estimation of Zolmitriptan and Sumatriptan succinate in bulk and Synthetic mixture.