The present study aimed to evaluate effects of Bryophyllum Pinnatum extract on calcium oxalate and calcium phosphate by in vitro methods. The leaves of Bryophyllum Pinnatum were sequentially extracted by using the hot maceration method, with a solvent such as ethanol, water and hydroalcoholic solution in various concentrations. The obtained extract was subjected to phytochemical screening and the test for alkaloid, flavonoid, saponin, phenol and triterpenoid saponin. For the in vitro study, experimentally calcium phosphate and calcium oxalate stones were prepared and compared with standard drug. Cystone is used as standard drugs. Bryophyllum Pinnatum is rich in phytochemicals such as alkaloids, saponin, glycoside, kamferol, and flavonoids and has a substantial capacity to dissolve calcium phosphate and calcium oxalate. These flavonoids inhibit calcium Phosphate and calcium oxalate deposits from forming in the renal tubules. The leaf extract contains anti-urolithiasis therapy and preventative capabilities and lowers the size of stones. In addition to diuretic and antiurolithic, antidiabetic, anticancer, anti-ulcer, anti-microbial, and wound healing activities, Kalanchoe pinnata leaf extract includes phenolic chemicals, tannin, and titerpenes. The main goal of the study is to find out how the Bryophyllum Pinnatum herb, especially its leaves, can prevent and treat health problems like renal stones, which are becoming more common in younger people because they don't exercise and eat poorly. The ability of the extract to get small particles out of the kidney and out of the urinary tract reduces the chance that they will get stuck in the urinary tract and form stones.