Quantitation of Ciprofloxacin by RP-HPLC from Active and Dosage Formulations

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A simple, sensitive isocratic reversed-phase HPLC method for detecting and quantifying ciprofloxacin in pharmaceutical dosage forms has been developed and validated according to ICH guidelines. In the method, the separation was achieved on a C₁₈ reversed-phase column with a mobile phase acetonitrile-water at a ratio of (80:20). The pH was adjusted to 2.7 using 85% of phosphoric acid with ultraviolet detection at 275.0 nm. A flow rate of 1.7 mL/minute was used at room temperature. The method's statistical evaluation was scrutinized through inter and intra-day precision assays, and it was found satisfactory through high accuracy and precision with a correlation coefficient of at least 0.9999. The quantification limit was 500 ng/mL, while this method's recovery was observed in the range of 99.01–101.19% and has the potential to be used for routine analysis in pharmaceuticals and clinical laboratories. The present method could also be useful to determine ciprofloxacin in human serum.