%0 Journal Article
%T Quantitation of Ciprofloxacin by RP-HPLC from Active and Dosage Formulations
%A Ahsan Zamir Siddiqi
%A Muneeba Akhtar
%A Agha Zeeshan Mirza
%J International Journal of Pharmaceutical And Phytopharmacological Research
%@ 2250-1029
%D 2022
%V 12
%N 5
%R 10.51847/jJ8O2TKNbn
%P 8-12
%X A simple, sensitive isocratic reversed-phase HPLC method for detecting and quantifying ciprofloxacin in pharmaceutical dosage forms has been developed and validated according to ICH guidelines. In the method, the separation was achieved on a C18 reversed-phase column with a mobile phase acetonitrile-water at a ratio of (80:20). The pH was adjusted to 2.7 using 85% of phosphoric acid with ultraviolet detection at 275.0 nm. A flow rate of 1.7 mL/minute was used at room temperature. The method's statistical evaluation was scrutinized through inter and intra-day precision assays, and it was found satisfactory through high accuracy and precision with a correlation coefficient of at least 0.9999. The quantification limit was 500 ng/mL, while this method's recovery was observed in the range of 99.01–101.19% and has the potential to be used for routine analysis in pharmaceuticals and clinical laboratories. The present method could also be useful to determine ciprofloxacin in human serum. 
%U https://eijppr.com/article/quantitation-of-ciprofloxacin-by-rp-hplc-from-active-and-dosage-formulations-yj7fq98av9c8hxn