TY  - JOUR
T1  - Quantitation of Ciprofloxacin by RP-HPLC from Active and Dosage Formulations
A1  - Ahsan Zamir Siddiqi
A1  - Muneeba Akhtar
A1  - Agha Zeeshan Mirza
JF  - International Journal of Pharmaceutical And Phytopharmacological Research
JO  - Int J Pharm Phytopharmacol Res
SN  - 2250-1029
Y1  - 2022
VL  - 12
IS  - 5
DO  - 10.51847/jJ8O2TKNbn
SP  - 8
EP  - 12
N2  - A simple, sensitive isocratic reversed-phase HPLC method for detecting and quantifying ciprofloxacin in pharmaceutical dosage forms has been developed and validated according to ICH guidelines. In the method, the separation was achieved on a C18 reversed-phase column with a mobile phase acetonitrile-water at a ratio of (80:20). The pH was adjusted to 2.7 using 85% of phosphoric acid with ultraviolet detection at 275.0 nm. A flow rate of 1.7 mL/minute was used at room temperature. The method's statistical evaluation was scrutinized through inter and intra-day precision assays, and it was found satisfactory through high accuracy and precision with a correlation coefficient of at least 0.9999. The quantification limit was 500 ng/mL, while this method's recovery was observed in the range of 99.01–101.19% and has the potential to be used for routine analysis in pharmaceuticals and clinical laboratories. The present method could also be useful to determine ciprofloxacin in human serum. 
UR  - https://eijppr.com/article/quantitation-of-ciprofloxacin-by-rp-hplc-from-active-and-dosage-formulations-yj7fq98av9c8hxn
ER  -