A rapid and precise reverse phase High-Performance Liquid Chromatographic method has been developed for the validation of Bisoprolol fumarate, in its pure form as well as in tablet dosage form. Chromatography was carried out on a sunsil C18 (150mm X 4.6mm, 5µ)column using a mixture of Acetonitrile: Water (60:40)v/v as the mobile phase at a flow rate of 0.8ml/min, the spectrometric detection was carried out at 223nm. The retention time of the bisoprolol fumarate was 1.990min respectively. The method produces linear responses in the concentration range of 4-14µg/ml of bisoprolol fumarate. The method precision for the drug bisoprolol fumarate was found to be within the limits. The method is useful in the quality control of bulk and pharmaceutical formulations.