In this study, the correlation between the displacement of the α-helices in cellular membrane channels and the frequency of the applied electromagnetic field was evaluated comparing the results after exposure to static, 50 Hz and 900 MHz electromagnetic fields of neuronal-like cells at the same intensity of 10 μT, using FTIR spectroscopy. The main result of this study was represented by the significant increase of the Amide I band, which was strictly related to the frequency of the field. Indeed, the integrated area of the Amide I band increased with increasing frequency, showing that the alignment of α-helices along the direction of the electromagnetic field is proportional to its frequency. This result can be applied in medicine, for instance, searching resonant natural frequencies of membrane channels in tumor cells and irradiating them by electromagnetic fields at those frequencies in order to damage their cellular functions. Also, it can be applied in modern technology, in order to plan technical wireless networks at frequencies far from resonant natural frequencies of biological systems.