International Journal of Pharmaceutical and Phytopharmacological Research
ISSN (Print): 2250-1029
ISSN (Online): 2249-6084
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2020   Volume 10   Issue 1

Protective Effects of Vitamin E on Mobile Phone Induced Injury in The Brain of Rats ‎

Sibghatullah Muhammad Ali Sangi1*, Abdulhakim Bawadekji2, Nawaf M Alotaibi1, Ahmed M. Aljameeli1, Samreen Soomro1

1 Faculty of Pharmacy, Northern Border University, P.O.Box840, Rafha-91911, Saudi Arabia.

2 College of Science, Northern Border University, P.O.Box 1321, Arar 91431, Saudi Arabia.

Aim: With increase in use of cell phone and exposure to radiation emission from Wi-Fi,cell damage in all the body systems is found.It is necessary to find ways and means to prevent that cell damage that may affect normal functioning of the organs etc. The objective of this study was to assess the damage to brain caused by exposure to cell phones connected with Wi-Fi and prevention of that damage with Vitamin  E. Methods: Thirty male Wistar Albino rats were used in the study,rats were divided in different groups, they were exposed to cell phones and Wi-Fi for 8 weeks. The rats were treated with Vit. E 50 IU/kg of bodyweight for 4 weeks.Results: Histopathological examination of the rat brain revealed that, exposure of rats to cell phones and Wi-Fi caused significant damage to the neurons in different areas of rat brain .The rats treated with Vit. E showed less damage in comparison to untreated rat groups. Conclusion: In the brain of rats, treated with Vit. E intact neuronal architecture was found  along with less inflammation. 

Key Words: Cell phone, Wi-Fi, Brain, Vitamin E, Antioxidant.



Brain is an organ that comprises a large mass of nerve tissues that are enclosed by skull. Major functions include sensory information processing, blood pressure regulation, breathing, and releasing hormones [1].

Brain damage is any harm that causes the destruction or impairment of brain cells. It occurs as a result of a wide range of internal and external factors. Brain damage can be defined as a significant, undiscriminating trauma-induced damage, while neurotoxicity typically is attributed to a selective, chemically-induced neuron damage [2].

Brain damage may occur via a wide range of conditions, illnesses, or injuries. Possible causes may include prolonged hypoxia (shortage of oxygen), poisoning, infection, neurological illnesses, and multiple traumatic injuries which can lead to chronic traumatic encephalopathy [3, 4]. And it has been investigated in various studies such as [5].

Oxidative stress is recognized as a main mediating process in the pathogenesis of cell damage and associated complications in different body systems, due to increased production of free radicals and abnormal antioxidant defense [6, 7]. It has also been found in previous studies that use of antioxidants (natural as well as synthetic ones) prevent cell damage in different organs due to diseases which enhance the oxidative stress or the direct insulting factors to the cells [8-10].

The brain damage caused by radiation depends on where the brain tumor is found, the quantity of radiation used, and the duration of the treatment. Radiosurgery can also lead to tissue damage that results in about 1 in 20 patients requiring a second operation to remove the damaged tissue [11, 12]. A study conducted by [13, 14], stated that there was no relationship between locations or regions of the brain exposed to radiations and brain tumor. It has also been concluded that there is a risk of acoustic neuroma as well as aglioma [15-17], and it increases with the increase in the cell phone use duration.

Nowadays, mobile or cell phone has become an important part of telecommunications in every individual’s life. In numerous countries, over half of the population use mobile phones. Although mobile phones are used by billions of individuals globally, a little increase within the incidence of adverse effects on health could have major public health implications on long term basis. Moreover, the number of cell phone calls every day, the length of each call and the amount of time people use phones are important factors which increase the health-related hazard [18].

Biological effects of electromagnetic radiation (EMR) emitted by a mobile phone have become a subject of an intense argument. A summary of the studies’ results was published lately by Kesari et al. [19] on which the authors conclude that the “regular and long term use of microwave devices, especially mobile phones can have a negative impact upon biological system, especially on brain, because the electromagnetic force source is held very close to the user’s head’’.

Vitamin E is a potent antioxidant soluble in lipids, preventing the propagation of free radicals and subsequent changes in membranes as well as plasmatic lipoproteins [20]. All forms of vitamin E have antioxidant activity [21]. The antioxidant effects of Vitamin E, which suppresses the free radical production and assists the neuroprotective effect have been reported by several studies [22-25].




Thirty Male Wistar Albino rats were obtained from the animal unit in faculty of Pharmacy, Northern Border University and used in the study, each weighing 240-260 g and 8 weeks old at the beginning of the experiment. They were housed in polycarbonate cages. They were kept in an environment of controlled temperature (25-26 <C), humidity (55-60%), and controlled light and dark period for 5 days before the start of the experiment. A standard balanced diet and tap water were provided.

Experimental Design:

The rats were randomized based on body weight into 5 groups prior to the administration as follows:

G1 was a vehicle control, and G2 was a treatment control exposed to mobile phone for 8 weeks. G3 and G4 were administered with Vitamin E 50 IU/kg of body weight for 4 weeks. These groups were exposed to mobile phones connected with Wi-Fi, for 24 hours a day for 8 weeks. G5 was exposed to mobile phones for 24 hours a day for 8 weeks. Whereas, G6 was administered with Vitamin E 50 IU/kg of bodyweight for 4 weeks.

All the animals were observed twice a day for signs of morbidity and mortality during the experimental period. Mobile phones were given calls after every 2 hours for 2 minutes.

Histopathological examination:

After removing the brain from skull, brain was sectioned sagittally. Right hemispheres were removed and fixed with a buffered 10% formalin solution for 24 h and embedded in paraffin. Tissues were then sectioned, stained with hematoxylin and eosin (H&E) and examined for histopathological changes using light microscope.



Group – 1Normal Filtered water (ad libitum)

The section from cerebellum shows partially distorted architecture consisting of focally intact molecular layer, granular layer [Fig.1, Arrow] and white matter [Fig.2, Arrow]. 

Figure 1. H&E x50


Figure 2. H&E x200Group – 3

Group – 3 EMR 900MHz (1hr/day) + Vit E

The section from cerebellum shows completely distorted architecture consisting of scanty molecular layer [Fig.3, Arrow]. The compact granular layer appears completely disrupted [Fig.4, Arrow]. The white matter cannot be assessed.

Figure 3. H&E x50


                                                                                     Figure 4.H&E x200


Group – 6   (Vit E)

The section from cerebellum shows partially distorted architecture consisting of focally intact molecular layer. The compact granular layer appears disrupted at places [Fig.5, Arrow] along with white matter [Fig.6, Arrow].



Figure 5. H&E x50


Figure 6. H&E x2


Cerebral Cortex

Group – 3 EMR 900MHz (1hr/day) + Vit E)

The section studied from the brain parenchyma shows partially preserved intact architecture. The pyramidal cells and neuroglial cells appear within normal limits [Fig.7, Arrow] with dense patchy inflammatory cell infiltration [Fig.8, Arrow]. The blood vessels appear unremarkable.


Figure 7. H&E x50

Figure 8.H&E x200


Group – 4EMR 900MHz (1hr/day) + Vit E)

The section studied from the brain parenchyma shows scanty preserved intact architecture. Some of the pyramidal cells and neuroglial cells show degenerative changes [Fig.9, Arrow] with sparse inflammatory infiltration [Fig.10, Arrow]. The blood vessels appear unremarkable.


Figure 9. H&E x50

Figure 10.H&E x200


Group – 2 EMR  900MHz (1hr/day) only

Sections from Hippocampus [Fig11., Arrow] shows distorted architecture consisting of cornuammonis [CA] and dentate gyrus. Stratum moleculare, Stratum pyramidale, Stratum radiatum, Granular cell layer and Fascia dentata appear distorted partially [Fig.12, Arrow]. Areas containing distorted cells are seen.


Figure 11. H&E x50


Figure 12. H&E x200

Group – 3 EMR 900MHz (1hr/day) + Vit E

The sections from Hippocampus [Fig.13, Arrow] show intact architecture consisting of cornuammonis [CA] and dentate gyrus. Stratum moleculare, Stratum pyramidale, Stratum radiatum, Granular cell layer and Fascia dentata appear within normal limits [Fig.14, Arrow].   


Figure 13. H&E x50


Figure 14. H&E x200


Caudate Putamen

Group-4: EMR 900MHz (1hr/day) + Vit E

The section from putamen shows completely distorted architecture with areas of disrupted white bundle matter containing few nuclei [Fig.15, Arrow] interspersed in completely disrupted neuropilfibers [Fig.16, Arrow].


Figure 15. H&E

Figure 16. H&E x200


Group-5: EMR 900MHz (1hr/day) + Vit. E (50 IU/kg)

The section from putamen shows partially distorted architecture consisting of focally preserved white bundle matter containing good number of deep nuclei [Fig.17, Arrow] interspersed in neuropilfibers [Fig.18, Arrow].


Figure 17. H&E x50


Figure 18.  H&E x200


The use of mobile phones is currently one of themost growing technological developments. The effect of radiation transmitted from mobile phones and other wireless electronic devices on human health is a subject of interest and study around the world, as a result of the enormous increase in mobile phone usage throughout the world [26]. The closeproximity of the antenna of a phone to theabdominal organs has increased the concerns about the biological interactions between electromagnetic radiation and body organs [27].

Although WHO stated that “A large number of studies have been performed over the last two decades to assess whether mobile phones pose a potential health risk”, To date, no evidence of adverse effects on health have been established as being caused by mobile phone use [28].

Hardel et al., and Lonn et al. [29, 30], concluded that exposure to mobile phone radiation depending on duration time  of mobile phone use, will result in increased possibility of incidence of brain tumors and acoustic neurinoma.

From the histopathological results of our study, it is evident that exposure to cell phones and Wi-Fi, produces significant inflammatory process in the different areas of brain and these findings are similar to the studies conducted by [31, 32]. Although the duration of the study was not very long, the damaging effects were observed. Continuous exposure may lead to prolonged inflammation in the brain leading to the cerebral edema, loss or impairment into the function of the organs and systems which are being controlled by the area in which damage occurs and increased risk of cancer due to repeated mutations in the area [33-35]. In this research, it was observed that standard antioxidant Vit E ,when used simultaneously with exposure to Wi-Fi and mobile phone, prevented the damage to brain cells and decreased inflammation was seen. These findings in the present study are consistent with the findings of [36] which concluded that vitamin E seems to be a highly promising agents for protecting endometrium tissue from oxidative damage and improving histopathologic changes caused by 900MHz EMR exposure which may alter the antioxidant capacity and catalase in rat brain [37]. The decrease in inflammation and damage to the nervous tissue can be attributed to a decrease in oxidative stress by Vitamin E which enhances nerve tissues protection by the removal of the free radicals and stabilization the cell membranes [38, 39].

It can be concluded that use of mobile phones and Wi-Fi cause inflammation and brain damage partly by increasing the free radical formation and partly by direct damage to the cells.

Conflict of interest:

The authors declares that there are no conflict of interest.


The authors wishe to acknowledge the approval and the support of this research study by the grant No (7725-PHM-2018-39-F) from the Deanship of  Scientific Research in Northern Border University (N.B.U.), Arar, KSA.



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