Pathogenic fungi found in the seminal fluid has been shown to have a negative influence on sperm quality. Aim: The present study investigated the mycological analysis of semen before the IVF procedure. Methodology: All samples were identified according to the morphological, biochemical, molecular, and antimicrobial tests. Results: fungal species were detected within 28% of semen samples and 72% did not show any fungal growth. The prevalent fungal isolates were Candida spp., followed byCandidaguilliermondii, Candida parapsilosis, and Candida glabrata. The use of mycological analysis to identify resistant fungal isolates is considered an effective method to reduce infertility in men by changing the contaminated sperm. Conclusion: Seminal fluid is not always sterile but it consists of diverse species of yeasts that are not related to totheIVF outcomes and a larger sample is needed to further examine our hypothesis. Also, microbiological examination of semen in men who have undergone failed IVF cycles may provide a chance to instigate antimicrobial therapy before the next conception.