Nanoparticles have risen rapidly to be one of the superior research scopes. The most suitable in the manufacture of nanoparticles as an alternative to the traditional physical and chemical methods are biological methods, bacteria provide many advantages in this context. The silver nanoparticles show interesting with compared to other salts due to widest applications in medical, therapeutics, and cosmetics. Reports of silver nanoparticle activity as antimicrobials are numerous, but the search on activity against the plant viruses was unpretentious. Diverse leave cultivars of tomato plants (Lycopersicon esculentum L) infected by Cucumber Mosaic Virus (CMV) are showing yellowing leaves, mottling, leaf curl, and leaf shoestring as a result of deformation. To better understand, the antiviral properties of silver nanoparticles are the efficacy of CMV disease control strategies. In this article, we will look at the effective role of silver nanoparticles as an excellent antiviral to CMV isolated from naturally infected tomato plants. Viral identity well confirms by direct ELISA test with antiserum to CMV, TMV, TEV, PVY, TSWV, PMMoV, and ToMV, host range, electron microscopy, and (RT-PCR) studies. The present study will evaluate the effects of exogenously applied silver nanoparticles on the percentage of CMV infection, disease severity, virus concentration, main photosynthetic pigments Phenolic compounds, and total protein and protein profile.