Cytotoxicity effect of 5-fluorouracil and bee products on the MCF-7 Human Breast Cancer Cell Line in vitro ‎

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Background: Cancer is a devastative disease in the world and uncontrolled cellular proliferation. Breast cancer is one of the most common causes of morbidity and 2nd cancer leading death among women worldwide. Current therapy available for cancer treatment is associated with the number of side effects. However, natural products offer an alternative route for the treatment of tumors. Objectives: The primary objective of our research to assess chemotherapeutic activities of 5-fluorouracil, natural bee products and combination doses against human breast adenocarcinoma cell line MCF-7 and screen cytotoxic effects of honey bee products against MCF-7 breast cancer cell lines. Materials and Methods: Samples of honey, Royal jelly, Pollen grains bee and their combinations without or with 5-FU, and tested by sulforhodamine B (SRB) cell-based assay against breast (MCF-7), cancer cell lines.Results: Treatment of in vitro MCF-7 breast cancer cell lines cultured cells with 5-FU alone resulted in an elevation in IC50 value (7.65uM/ml,), compared with one or more of bee products (IC50>100uM/ml). Contrary, treated by 5-Fu and with one or more of Honey (RJ, H, PG&Mix), led to a decrease in IC50(7.65; 4.95; 23.45 and 4.26 uM/ml). Combination with honey products such as RJ, H, PG led to a deficiency of cell viability and capability of 5-FU to effect MCF-7 growth of breast cancer in vitro only during treatment supplementation with bee products. The high dose-dependent effect in cell viability tests. Conclusion, Combinations of one dose of Royall jelly, honey, pollen grains or its combinations with 5-FU five suppressive actions on the viability of MCF-7 breast cancer cell lines in contrast to 5-FU (same dose). Bee products (RJ, H&PG) have synergistic cytotoxic effects with 5-FU in MCF-7 breast cancer cell lines in vitro.