Green synthesis of silver nanoparticles (AgNPs) was achieved by the bio-reduction of silver nitrate using Walsura trifoliata plant aqueous leaf extract. The AgNPs are characterized by UV-Vis, FT-IR, and TEM. The AgNPs formation was preliminarily confirmed by Ultraviolet-Visible (UV-Vis) spectrophotometer. The synthesized AgNPs in the solution has shown a lesser absorption at 434nm. The Fourier-Transform Infrared (FTIR) Spectroscopy was used to validate the presence of several functional groups responsible for decreasing and stabilizing during the biosynthesis process. The TEM is used to know the morphology, size, and agglomeration pattern of the nanoparticles. The characterization of silver nanoparticles revealed a spherical crystallite nature with particle sizes ranging between 21.08-24.91nm, the average size of the nanoparticles 22.48nm. The synthesized AgNPs showed remarkable antimicrobial activity against clinically isolated two Gram-negative bacteria (K. pneumoniae and E.coli) and two Gram-positive bacteria (B. subtilis and S. aureus); and antioxidant (2, 2-diphenyl-1-picryl-hydrazyl (DPPH), H2O2 and NO radical scavenging activities.