A project was designed to investigate the effect of extraction solvents on phytochemical extraction of plants endemic to the desert habitat of Cholistan Desert, Pakistan. In this study four types of solvents Chloroform, Acetone, Propanol, and Acetic acid were used to examine total antioxidant activity contents, total flavonoid contents, and total tannin contents. Plants typically of the desert area were selected. Keeping in view the similar age of plant and their parts the specimens were collected with three replicates of each. Specimens after proper identification and labeling were processed for extraction. Results showed that extraction solvents had significant differences for extraction of total antioxidant activity contents, total flavonoid contents, and total tannin contents. Acetone showed maximum total antioxidant activity contents and Chloroform showed maximum total flavonoid contents. Minimum total antioxidant activity contents and total flavonoid contents were measured by extraction in Acetic acid. Maximum total tannin contents were measured by solvent Propanol.