Background: Radiographic imaging is extremely valuable as a diagnostic tool in medicine, but ionizing radiation and computed tomography (CT) scans carry well-known potential risks, which can lead to serious health hazards to healthcare workers (HCWs) and the nearby environment if safety measures are not properly applied. Objective: To assess the knowledge and awareness among Saudi Medical interns in Eastern Providence regarding radiation protection and radiological examination doses. Result: We recruited 387 Saudi medical interns between two universities (KFU: 202; IAU: 185), The proportion of medical interns who attended radiation protection courses, who were having adequate information about radiation risk and radiation protection measures were 11.1%, 43.2%, and 31%, respectively. When comparing KFU and IAU, those who attended radiation protection courses (p<0.001), those with having adequate information about radiation risk (p=0.002), and those with adequate information about radiation protection measures (p<0.001) were statistically significantly higher at KFU. Conclusion: The present study found that medical interns in KFU and IAU lack sufficient knowledge concerning radiation, radiation protection, health risks, and doses used for radiological applications.