Resistance to antibiotics is increased in all hospitals, and bacterial pathogens that contaminate burns and wounds are widespread. Their resistance to well-known antibiotics is recorded, and every day resistant isolates are counted and studied. The present study aimed to prepare new polymers with antimicrobial activities. The effect of some polymers prepared from chitosan and collagen associated with nanoparticles of Cu+, Ti4+, and Ag+ was determined using some bacterial pathogens as test organisms. The tested bacteria were two gram-negative, Pseudomonas aeruginosa and Escherichia coli and four gram-positive, Enterococcus faecalis, Streptococcus sp., Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus [MRSA]. The antimicrobial activity of the prepared polymers was determined using three methods; diffusion method, colony-forming unit [CFU] and Optical density method. The beetroot plant was extracted by boiling water to obtain the water extract which was used to prepare the polymers. The results showed that all nanocomposite films were active against different pathogenic bacteria. The antibacterial activities of the discs of four different prepared films were compared. The most active film was Chitosan-Collagen nanocomposite with three different metals. Gram-negative Pseudomonas aeruginosa and Gram-positive MRSA were the most sensitive bacteria in incorporating films. In conclusion, films of chitosan, collagen, and nanoparticles [CuO, TiO2, AgNO3] had excellent antibacterial abilities against both Gram-positive and negative bacteria. Thus, they can be used as dressing in wounds and burns for healing.