Introductıon: Anxiety is the most common emotional reaction of women during childbirth and can lead to adverse effects on fetuses and mothers. Women experience severe anxiety during childbirth, which reduces their chances of giving birth. Therefore, the aim of this study was to evaluate the effect of Jacobson relaxation on reducing the level of women anxiety in active phase of childbirth. Materials and Methods: This clinical trial study was performed on 90 pregnant women after informed consent, by random sampling in an intervention group (group for which relaxation was performed) and a control group. At first Eshpel Burgers level of anxiety was measured and then for 20-30 minutes the relaxation was performed for the intervention group and then again, the level of anxiety was measured by the Eshpel Burgers questionnaire. Data analysis was done using SPSS 20 software. Findings: Levels of anxiety before and after intervention were the same in both groups. The level of anxiety after relaxation in the intervention group was significantly lower than the control group (p <0.001). Conclusion: The results of this study showed that the use of complementary medicine in the method of Jacobson muscle relaxation is inexpensive, safe and effective in childbirth, which can be effective in reducing anxiety and is the basis for further research.