The nutritional status can be determined by serum proteins. Normal protein levels being off-balance signals possible life-threatening organ malfunction. The study's primary goal was to ascertain the serum and tissue protein levels in response to CCl4 intoxication and their reduction by a newly created innovative herbal medicine (SS-IM-21). Swiss albino adult healthy mice were divided into four groups of six each based on weight. Group I served as the control, Group II was given Multi herbal formulation (SS-IM-21) 400 mg/kg/day, Group III was given carbon tetrachloride (CCl4) 1 ml/kg-bw, and Group IV was given CCl4 combined with SS-IM-21 (400 mg/kg). In contrast to untreated animals, therapy with Multi herbal formulation (SS-IM-21) normalized body weight, food intake, and water intake in mice after administration of carbon tetrachloride (CCl4). When compared to the control, Group III receiving CCl4 treatment saw lower serum levels of total protein, albumin, and globulin. In comparison to CCl4-impaired animals, pre-treatment with SS-IM-21 significantly (p<0.001) raised the levels of total protein, albumin, and globulin in serum, liver, and kidney. However, a greater albumin/globulin ratio makes it evident that the liver and kidney may be negatively impacted by CCl4 medication. By preserving the albumin/globulin ratio, the multi-herbal formulation (SS-IM-21) safeguards the kidney and liver. In conclusion, it is possible to predict that antioxidant-enhanced new formulation with phytochemicals preserve normal protein patterns and shield the organism from a variety of dysfunctions.