Molecular Study for the Virulence Factor of Entamoeba spp by Gene Sequence Technique

Download PDF

Amoebiasis is characterized as one of the invasive extraintestinal or intestinal infections. The current study used PCR for 296 microscopically positive patients. The molecular study showed that of 144 (48.64%) were Entamoeba (E.) histolytica, 91(30.74%) were E.dispar and 52(17.56%) were E.moskovski. The association of E. histolytica and E. dispar was recorded for 9(3.04%). The positive samples with PCR have been submitted to detect the virulence factors of E.histolytica by PCR and the results showed that 61 samples (42.36%) were positive with cysteine proteases gene, 50 (34.72) were positive with amoebapore and 33(22.91) were positive with lectin. The gene sequence for cysteine protease, amoebapore, and lectin in local E. histolytica human isolates showed the nucleotide alignment similarity and substitution mutations in all of the cysteine protease, amoebapore, and Gal/GalNAc lectin. Phylogenetic tree analysis based on cysteine protease gene partial sequence in local E.histolytica human isolates that used for genetic variation analysis showed genetic closed related to NCBI-BLAST E.histolytica  strain (M27307.1) at total genetic changes (0.0020-0.0050%), while phylogenetic tree analysis based on amoebapore B gene partial sequence in local E.histolytica human isolates that used for genetic variation analysis showed genetic closed related to NCBI-BLAST E.histolytica,  strain (MS30-1047) at total genetic changes (0.20-0.050%). Phylogenetic tree analysis based on Gal/GalNAc lectin gene partial sequence in local E. histolytica human isolates that were used for genetic variation analysis showed genetic close related to NCBI-BLAST E.histolytica HM-1:IMSS strain (AP023115.1) at total genetic changes (0.0080-0.0020).