The genus Streptomyces, known as prolific antibiotic products and many other bioactive secondary metabolites, is a unique subgroup of actinomycetes bacteria. This study aims to isolate Streptomyces strains from plant rhizosphere that has antibacterial activity. Soil samples were collected from Saudi Arabia. About 39 isolates of Streptomyces sp. were obtained from 12 soil samples, using serial dilution and plating techniques on the solid starch nitrate medium. The Streptomyces strains were cross streaked against some human pathogen bacteria. Against various pathogenic bacteria, only 16 out of 39 isolates demonstrated strong antimicrobial activity. The most active strain Streptomyces NM38 was chosen for secondary screening and identification. The molecular identification was confirmed using 16S rRNA as Streptomyces globosus, the phylogenetic tree was built; the sequence was presented to the GenBank under the accession number: MN538259.1.