Accurate echocardiographic assessment of cardiac function and establishing a diagnosis requires knowledge of normal echocardiographic indices. Although several studies have addressed the normal echocardiographic values in different breeds of dogs and cats, no study has specifically measured these variables in a population of Persian cats. We performed two-dimensional and M-mode echocardiography on a total of 76 healthy, adult Persian cats without sedation. The 16 echocardiographic indices were then compared between male and female cats. Our findings indicate a significant difference in end-diastole left ventricular free wall thickness between females and male Persian cats (female: 6.38 vs male: 7.11 mm; p-value = 0.01). Also, the heart rate, end-diastole left ventricular free wall thickness, and end-diastole left ventricular volume were lower in females compared to males, although the difference was barely significant (all p values = 0.05). Considering the popularity of Persian cats as pets and their susceptibility to cardiomyopathy diseases, the findings of this study may be used as a basis for further research on abnormal cardiac conditions in this breed of cats.