Objective: The present study aimed to evaluate the in-vitro antioxidant activity of Hordeum vulgare belonging to family Poaceae. Methods: The shade-dried stem part of H. vulgare (1kg) was powdered and extracted by chloroform, petroleum ether, ethanol, and aqueous extraction methods using soxhlation. The extracts were concentrated using a rotary evaporator under decreased pressure at 40 °C until they were free of solvents. Thereby crude extracts were provided and employed for further studies. The antioxidant activity of Hordeum vulgare leaf using DPPH* radical scavenging model and to assess the antioxidant activity of Hordeum vulgare leaf and stem using Nitric oxide free radical (NO*) scavenging model and to assess the antioxidant activity of Hordeum vulgare leaf using superoxide free radical (SO*) scavenging model and to assess the antioxidant activity of Hordeum vulgare leafusing hydroxide free radical (OH*) scavenging model.Results: The graph was extrapolated between different concentrations of the plant extracts and the inhibition percentage to find out the half-maximal inhibition concentration. The extracts exhibited dose-dependent neutralization of DPPH*, NO*, SO*, and OH* free radicals and their activity was compared with standard curcumin. The IC50 was calculated for 310 µg, 620 µg, and more than 640 µg/ml of the ethanolic extract of Hordeum vulgare stem against DPPH*, NO*, SO*, and OH* free radicals, respectively. This indicated that the ethanolic extract of Hordeum vulgare leaf exhibited antioxidant activity.Conclusion:The antioxidant activity was exhibited due to the presence of tannins, flavonoids, and phenolic compounds, which were present in the methanolic extract of Hordeum vulgare.