Background & objectives: Chemical drugs are controlling diabetes mellitus and not effective for the absolute cure. By emphasis on traditional medicine and considering the antioxidant property of Prosopis farcta containing the phenol and flavonoid components, this study was conducted to evaluate the effect of hydro-alcoholic and aqueous extract of P. farcta on the glucose uptake level in 3T3-L1 adipocytes. Methods: Recent in vitro study with cells which were grown with Dulbecco's Modified Eagle Medium (DMEM) and were divided into control group, positive control, negative control group by preventing glucose uptake stimulation with 1 µM Cytochalacin D and treatment groups containing glucose uptake stimulation with aqueous and hydroalcoholic extracts of P. farcta at a different dosage. To measure the amount of glucose uptake by cells, the instruction of the Abcam glucose uptake kit was used. Results: Doses of 160 µg/mL of hydro-alcoholic and 800 µg/ml of the aqueous extract of P. farcta have the maximum amount of 2- deoxyglucose uptake in adipocyte cells (p<0/001). Between 800 µg/mL dosage comparison, the aqueous extracts had the most pronounced effect (p<0/001). Interpretation & conclusions: Findings showed that both aqueous and hydroalcoholic extract of P. farcta in a dose-dependent manner have been effective in increasing the level of glucose uptakes into the 3T3-L1 adipocytes. However, future studies must find possible molecular pathways associated with P. farcta action.