In the present investigation, the zinc oxide (Zno) and copper oxide (CuO) nanoparticles were synthesized and characterized by U.V. studies, SEM, particle sizer, FTIR studies, zeta potentiality, and antioxidant activity. The SEM images of Zno NP showed circular beaded structures. The Cuo nanoparticles seemed to be berry shaped. These NPs were further evaluated for antimicrobial activity. The antimicrobial studies were preferably conceded by using cup plate method against a standard antibiotic. These studies revealed that Zno and Cuo NP were able to show antimicrobial activity against both Gram-positive and Gram-negative bacteria, and hence can be used to control antibiotic-resistant bacterial pathogens. The nanoparticles were able to show antimicrobial activity because of their nanosize and disinfectant activity.