The patient number pursuing alternative and herbal treatments is enormously rising. Herbal medicines are the therapeutic experiences mixture of practicing generation's physicians of indigenous medicine organizations for over hundreds of years. Herbal medicines are now in countless mandates in developing nations for primary healthcare not for the reason that they are reasonably priced and for improved cultural adequacy, enhanced compatibility with the human body. Hence, the study objective is to comprehend the consequence of diverse levels of (Vitex Agnus-Castus) plant on the Histopathological and Immunological alterations in rats injected with carbon tetrachloride (Ccl4). The experiment was carried out in an animal house. All rats were provided for a week on a basal diet before starting the trial, then separated into 2 primary groups; the first group (n= 6 rats) was provided only as a negative control on the basal diet (C –ve) normal rats for 28 days. The rats of the second leading group (n= 24 rats) were injected with carbon tetrachloride (Ccl4). The second leading group is divided into four sub-groups, including three groups fed with different concentrations of Vitex Agnus (5%, 10%, and 15%) and one group control positive infected with the disease did not provide on the experimental diet. The outcomes showed serum IgG insignificant changes between 15%, 10%, and 5% Vitex Agnus-Castus compared with negative control. In contrast, serum GPX showed substantial modifications between 10% and 15% Vitex Agnus-Castus than the negative control. In this study Vitex Agnus is used to improve the immune system.