TY  - JOUR
T1  - Effect of CpG S ites on Transcription Factor in Promoter of ‘RASA3’ Gene
A1  - Khloud Algothmi
A1  - Hadeel Kalboneh
A1  - Aisha Alrofaidi
A1  - Mona Alharbi
A1  - Reem Farsi
A1  - Najla Alburae
A1  - Magdah Ganash
A1  - Alawiah Alhebshi
A1  - Fatemah Basingab
A1  - Sheren Azhari
A1  - Manal Shaabad
A1  - Ashraf Dallol
A1  - Amany Alqosaibi
A1  - Mohammed Jan
A1  - Hesham Aldhalaan
A1  - Safiah Alhazmi
JF  - International Journal of Pharmaceutical And Phytopharmacological Research
JO  - Int J Pharm Phytopharmacol Res
SN  - 2250-1029
Y1  - 2021
VL  - 11
IS  - 1
DO  - 10.51847/80ckl5vNEC
SP  - 120
EP  - 124
N2  - RAS p21 activator 3 gene is a member of the Ras GAP1 family of GTPase-activating proteins. DNA methylation is the most well-known epigenetic mechanism. Abnormality on RAS proteins which are regulated by RASA3 may play a critical role in Autism. Autism is a complex of neurodevelopmental disorders. Defining the regulators of RASA3 is important to understand its role in Autism. This study included 19 Saudi autistic children and their healthy siblings. Blood was used to analyze the differential expression of RASA3 and its transcriptional factor Specificity protein 1. qRT-PCR and MethyLight assay was used to measure CpG sites methylation on TFBs  in the promoter of RASA3 to investigate the association between methylation level and TF in the regulation of RASA3 expression. Results showed differential expression patterns for both RASA3 and Sp1 genes with a significant positive correlation between them. There was no difference in RASA3 methylation level in such binding sites of the target region in the promoter of RASA3 in autistic and healthy samples. The data suggested that the Sp1 could play a critical role in the regulation of RASA3 expression while DNA methylation does not. However, it remains an open question whether the dysregulation of RASA3 by methylation in other regions may be implicated in the development of ASD.
UR  - https://eijppr.com/article/effect-of-cpg-sites-on-transcription-factor-in-promoter-of-rasa3-gene
ER  -