The present research reflected towards exploring the plausible role(s) of the active extracts of Tridax procumbens (whole plant), Murraya koenigii (leaves), Calendula officinalis (flowers), and Aloe barbadensis (leaves) formulated as ointment products. The formulations were characterized by determining the pharmaceutical characteristics like skin irritancy test, pH, appearance, viscosity, spreadability, extrudability, swelling index, and washability. Further, wound healing activity was studied on Swiss albino rats. The phytochemical constituents such as alkaloids, flavonoids, glycosides, tannins, carbohydrates, sterols, saponins, proteins, and other miscellaneous phenolic components are believed to play a pivotal role in the healing of the wound in rats by significantly increasing the rate of wound closure and rate of epithelization. In comparison to the standard drug (betadine), both the formulations proved to be quite equi-efficacious. This finding provided an insight into the applications of the polyherbal formulations in the traditional treatment of serious wound conditions and also rejuvenating the ethnopharmacological principles in context to modern medicine.