Herbal products have been employed by humans as a medicine for various ailments. The main goal of this research is to design a polyherbal dosage form to treat malaria effectively as well as to modulate the immune response. The basic behind choosing herbs for formulation development was a better treatment option with minimal side effects. Three herbs were taken for the development of formulation; these are Nyctanthes arbor, Tinospora cordifolia, Ocimum sanctum. The formulation was developed in the form of vati as per the convenience of administration to the patient. Results of research conclude that the minimum inhibitory concentration of designed formulation was found 0.98 µg/ml, where chloroquine and quinine were used as standard drugs and the minimum inhibitory concentrations were found to be 0.020 µg/ml and 0.268 µg / ml respectively. All the experimental work suggested that the polyherbal formulation developed can be another effective treatment option for malaria.