Certain Extracellular Productions in Bacillus subtilis Cultures Supplemented with Banana Waste as Substrate

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The Bacillus subtilis (k1) grew in submerged fermentation cultures supplemented with various organs of banana agricultural wastes as the cheapest substrate. Due to the differential richness of cellulose, hemicellulose, and lignin of banana organs, it could be a valuable factor for B. subtilis growth and the production of valuable secondary products. The B. subtilis was inoculated on LB0 (TY-medium), LB1 (⅛ TY-medium), LB2 (LB1 + leaf blade), LB3 (LB1 + leaf midrib), LB4 (LB1 + leaf sheet), LB5 (LB1 + cobe leaflets), LB6 (LB1 + fruit peel) and LB7 (LB1 + sucker) cultures supplemented with 15 % and 30 % extracts of each banana organ. After 12th hrs of culture incubation (37°C, 250 rpm). All Bacillus cultures have shown variant growth rates except LB1. Maximum reducing sugars were observed on LB2a (0.442±0.041 mg ml-1), LB4a (0.444±0.012 mg ml-1) and LB6 (0.444±0.007 mg ml-1), while total proteins on LB3a and LB5a. Meanwhile, free prolines and glycine betaine observed maximum in the LB2 and LB3a cultures respectively. The highest enzyme activities were also noted as pectinase in LB6a, amylases in LB7a, cellulases LB2a, lipase LB4, and proteases LB7, while pectinases and lipases remained maximum over others among the cultures at the harvest time (p≤0.05). These findings have shown that each organ of banana waste has different fueling potential, which could be a useful substrate for the biosynthesis of hydrolytic industrial enzymes with bacterial fermentation