TY  - JOUR
T1  - Antimicrobial and Antioxidant Compounds Produced by Fungal Endophytes Isolated from Selected       Nigerian       Medicinal Plants
A1  - David Chinemerem Nwobodo
A1  - Malachy Chigozie Ugwu
A1  - Melford Chuka Egbujor
A1  - Festus Basden Chiedu Okoye
A1  - Charles Okechukwu Esimone
JF  - International Journal of Pharmaceutical And Phytopharmacological Research
JO  - Int J Pharm Phytopharmacol Res
SN  - 2250-1029
Y1  - 2023
VL  - 13
IS  - 3
DO  - 10.51847/7hNZ3qg4m4
SP  - 6
EP  - 15
N2  - Endophytic fungi can inherently establish mutual relationships with host plants while also synthesizing similar and enhanced bioactive compounds as the host. The investigation of exploitable antibacterial and antioxidant bioactive compounds produced by fungal endophytes from selected medicinal plants was the focus of this study. Following standard procedures, 15 fungi were isolated from Cola acuminata, Bambusa vulgaris, and Elaeis guineensis leaf segments. By solid-state fermentation using a rice medium, fungal secondary metabolites were extracted using ethyl acetate. About six human pathogens, including Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Aspergillus niger, and Candida albicans, the antibacterial properties of the crude extracts were assessed using the agar diffusion assay. Except for E. coli, all endophytic fungal extracts showed promising inhibitory effects against all test isolates. The free radical scavenging activity was estimated using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical assay, and the bioactive constituents were analyzed using Gas Chromatography-Mass Spectroscopy. The metabolites of the endophytic fungi also showed good antioxidant activity. Three of the fungi with the best activities were identified as L. theobromae (Ca1), C. lunata (Bv4), and C. lunata (Eg7), using molecular techniques (ITS region). GC-MS analysis of these fungal extracts revealed the presence of 17 antimicrobial and antioxidant compounds such as 2,4-di-tert-butylphenol, p-Cymene, γ-Terpinene, β-Bisabolene, and hexadecanoic acid, among others. Endophytic fungi associated with Nigerian medicinal plants are potentially rich sources of antibacterial and antioxidant compounds that could be exploited for drug discovery. 
UR  - https://eijppr.com/article/antimicrobial-and-antioxidant-compounds-produced-by-fungal-endophytes-isolated-from-selected-n-sbmlbvdpmg5ukap
ER  -