Background: The present study was conducted to determine the most prevalent isolates in urine cultures and resistance profile of the isolated pathogens and trend of changing resistance to common antibiotics used for the treatment of urinary tract infections. Materials and methods: In this longitudinal - descriptive study, the data of 2060 positive urine cultures and the susceptibility testing results of inpatients and outpatients’ urine specimens from 2007 to 2014 were studied. The susceptibility testing using Kirby-Bauer disk diffusion method according to CLSE standards was done on isolated uropathogens for t ceftriaxone, ceftazidime, gentamicin, amikacine, ciprofloxacin and cotrimoxazole, pipracillin, nitrofurantoin and imipenem. Results: The most prevalent pathogen which was isolated was E.coli, 1141 (55.4%). The resistance trend of E.coli isolates for antibiotics showed increased resistance for: ceftazidim (67% to 81%), ciprofloxacin (57%-77%), pipracillin (20%-87%): stable resistance rate for : ceftriaxone(73%-70%), cotrimoxazole (79%-74%) and decreased resistance for : nitrofurantoin (32%-8%), gentamicine (49%-37%) and amikacine (21%-13%). 50% of E.coli isolates were co-resistant to ceftriaxone and ciprofloxacine and trimetoprim-sulfamethoxazole. Conclusion: Antibiotic resistance of uropathogens for most of the important antibiotics empirically used for UTI was very high and increasing in this hospital.